MATERIALS AND METHODS
SAMPLE COLLECTION
Soil samples were (5 of each location) collected from different habitats like forests, agricultural fields (cultivated and un cultivated), hills and gardens form, Bidar, Raichur, Bijapur, Koppal and Gulbarga. All soils were of laetrile type. Soil samples were dried under room temperature for 15-30 days, before isolation

Table:1. Characteristic features of soil Samples
Sl.No Soil type Location Habitat Collection Laboratory
1 Black Gulbarga Alkaline Apr 2004 DAS 69
2 Red Bidar Alkaline Apr 2004 DAS 123

3

 Black Bijapur Alkaline May 2004 DAS 131
4 Black Raichur Alkaline May 2004 DAS 135
5 Red Gulbarga Salt Apr 2004 DAS139
6 Black Koppal Alkaline June 2004 DAS143
7 Black Gulabrga Alkaline June 2004 DAS147
8

Black

 Gulabrga Alkaline July 2004 DAS 165
9 Black Gulbarga

Alkaline

 July 2004 DAS 178

ISOLATION OF PIGMENTED MICROORGANISMS
The pigmented organisms preferably Actinomycetes had been isolated by pour plate technique on starch-casein agar and Glycerol-argenine agar, after serial dilution in distilled water. dry colonies  of pigmented Actinomycetes were selected and isolated. Thus isolated colonies had been preserved in Glycerol based media and stored at 20ºC.

 BIOCHEMICAL      AND     PHYSIOLOGICALCHARECTERIZATION OF ACTINOMYCETES
The potent Actinomycetes selected from screening were characterized by morphological and biochemical methods. Morphological methods consist of macroscopic and microscopic methods. The microscopic characterization was done by cover slip culture method, the mycelium strcture, colour and arrangement of conidiophores and arthospores on the mycelium were observed under scanning electron microscope. The observed structure was compared with Bergey’s manual of Determinative Bacteriology (1989) and the organism was tentatively identified.

Various biochemical tests are performed for the identification of the potent isolates, like casein hydrolysis, starch hydrolysis, cellulose hydrolysis, pectin hydrolysis, urea hydrolysis, and melanin formation. sugar fermantation, Nacl resistance and temperature tolerance.

Corresponding Author:
Dr.A.Dayanand, Reader.Dept.of.Microbiulogy,
Gulbarga University Gulbarga-585 106,Karnataka, India.
Phone:91-08472-265837
E-mail: iamdaya62@rediffmail.com
   

© Dr.A.Dayanand