 |
|
|
RESULTS AND DISCUSSION
The 9 typically pigmented Actinomycetes were
screened and isolated from total 180 different sites of Gulbarga region. Most of
the isolates were confirmed that they belong to the genera of Streptomyces, as
they showing good sporulation with compact, dry, powdery colonies with typically
pigmentation.
DISCUSSION
The result of screening reveals that most
of the pigmented microorganisms are probably of Actinomycetal group. All strains
grow at 3 % of Nacl and all are them tolerate up to 7% of Nacl in media. They
hydrolyses the 25-30% of casein and starch in a wide range of pH and they
tolerate up to 42șC.And
further standardization of bioprocess technology is under process to study the
parameters for the production aspects of Amylase and Protease through
solid-state fermentation from a cheap agro waste as a substrate.
Fig 2. Pigmented Isolates of Actinomycetes Detected
 |
 |
 |
|
DAS 69 |
DAS 123 |
DAS 131 |
|
|
|
 |
 |
 |
|
DAS 135 |
DAS 139 |
DAS 143 |
|
|
|
 |
 |
 |
|
DAS 147 |
DAS 165 |
DAS 178 |
|
|
|
Table:2. Biochemical and Physiological
Characteristics.
|
Characters |
DAS 69 |
DAS 123 |
DAS 131 |
DAS 135 |
DAS 178 |
DAS 143 |
DAS 147 |
DAS 165 |
DAS 139 |
|
Hydrolysis |
|
|
|
|
|
|
|
|
|
|
Casein |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Starch |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Cellulose |
+ |
-- |
+ |
-- |
+ |
+ |
+ |
+ |
+ |
|
Pectin |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
-- |
|
Urea |
-- |
+ |
-- |
+ |
-- |
-- |
-- |
-- |
-- |
|
Nitrate Reduction |
-- |
+ |
-- |
+ |
-- |
-- |
-- |
-- |
-- |
|
Melanoid |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
|
Temperature |
|
|
|
|
|
|
|
|
|
|
25șC |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
37șC |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
42șC |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Nacl (%) |
|
|
|
|
|
|
|
|
|
|
3 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
7 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
10 |
-- |
+ |
- |
+ |
-- |
-- |
-- |
-- |
-- |
|
PH |
|
|
|
|
|
|
|
|
|
|
6 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
8 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
10 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
12 |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Sugar utilization |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Reffinose |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Rhamnose |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Galactose |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Sucrose |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Arabinose |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Fructose |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Xylose |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Mannitol |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
|
Inositol |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
+ |
Table:3. Color of Aerial
Mycelium of Isolated Strains
|
Isolates No |
Color of Mycelium |
Medium Used |
|
DAS 69 |
Dark Blue |
SAC/GAA |
|
DAS 123 |
Dark Red |
SAC/GAA |
|
DAS 131 |
Dark Pink |
SCA/GAA |
|
DAS 135 |
Red |
SAC/GAA |
|
DAS 139 |
White |
SCA/GAA |
|
DAS 143 |
Light Pink |
SAC/GAA |
|
DAS 147 |
Ash Color |
SCA/GAA |
|
DAS 165 |
Cement Color |
SAC/GAA |
|
DAS 178 |
Brown |
SAC/GAA |
SCA/GAA: Starch-casein agar and Glycerol argenine
agar
Fig 3. Spore Structure of Isolated Actinomycetes
 |
 |
 |
|
DAS 69 |
DAS 123 |
DAS 131 |
|
|
|
 |
 |
 |
|
DAS 135 |
DAS 139 |
DAS 143 |
|
|
|
 |
 |
 |
|
DAS 147 |
DAS 165 |
DAS 178 |
|
Corresponding Author:
Dr.A.Dayanand, Reader.Dept.of.Microbiulogy,
Gulbarga University Gulbarga-585 106,Karnataka, India.
Phone:91-08472-265837
E-mail: iamdaya62@rediffmail.com |